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HSD3B7 (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of HSD3B7 in human plasma, tissue homogenates and other biological fluids.
Catalog #: E4815
$725.00

Product Details

Cat # +Size E4815-100
Size 96 assay
Detection Method Sandwich ELISA, Absorbance (450 nm)
Species Reactivity Human
Applications • A Sandwich ELISA kit for the quantitative measurement of HSD3B7 in human plasma, tissue homogenates and other biological fluids.
Features & Benefits • Sensitivity: 0.094 ng/ml
• Detection Range: 0.156-10 ng/ml
• Precision: Intra-Assay: CV<8%, Inter-Assay: CV<10%
Kit Components • Micro ELISA Plate
• Lyophilized Standard (10 ng)
• Sample / Standard dilution buffer
• Biotin- labeled antibody
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

HSD3B7 encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein 3 beta-hydroxysteroid dehydrogenase type 7 is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in HSD3B7 are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. BioVision’s HSD3B7 (Human) ELISA Kit is based on the Sandwich ELISA principle. Capture antibody was precoated onto 96-well micro ELISA plate and the biotin conjugated antibody was used as detection antibody. The standards, test samples, and biotin conjugated detection antibody were added to the wells subsequently, incubated and followed by washing. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding the acidic stop solution. The density of color is proportional to the amount of HSD3B7 captured from the samples.


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