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HRV 3C Protease Activity Assay Kit (Colorimetric)

Industry's first assay kit to measure HRV 3C Protease Activity.
Catalog #: K214
$475.00

Product Details

Cat # +Size K214-100
Size 100 assays
Detection Method Absorbance (OD= 405 nm).
Applications Detection of HRV 3C Protease activity in samples
Determine activity of purified HRV 3C Protease
Features & Benefits • Simple, rapid assay
• Can detect HRV 3C Protease activity as low as 50 ng in samples
Kit Components • HRV 3C Protease Assay Buffer
• HRV 3C Protease (10 μg, Positive Control)
• HRV 3C Protease Substrate
• pNA Standard (0.1 M)
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes. BioVision’s HRV 3C Protease Activity assay kit utilizes the ability of a 3C Protease (derived from a HRV rhinovirus-14, EC: 3.4.22.28) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. This assay kit is simple, rapid and can detect HRV 3C Protease activity as low as 50 ng in samples and of purified proteins.


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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Hanna J. Wagner, Biofunctionalized Materials Featuring Feedforward and Feedback Circuits Exemplified by the Detection of Botulinum Toxin A. Adv Sci (Weinh), Nov 2018;  30828524.
For more citations of this product click here