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HMGA2 (Human) ELISA Kit

Sandwich ELISA kit for quantitative measurement of HMGA2 in human serum, plasma, tissue lysates, and other biological fluids

WARNING:
This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California
to cause cancer. For more information go to www.p65warnings.ca.gov/.
Catalog #: E4960
$695.00

Product Details

Cat # +Size E4960-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit to quantitatively measure HMGA2 in human serum, plasma, tissue lysates and other biological fluids
Features & Benefits ● Detection range: 0.156 – 10 ng/ml
● Sensitivity: 0.094 ng/ml
● Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
● This Sandwich ELISA is highly sensitive and highly specific for the detection of HMGA2 in human samples. There is no significant cross-reactivity or interference between HMGA2
● and analogues
● Recovery range: 85 - 105% for normal human serum and plasma samples
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate Sealers
● Standard (Lyophilized) (10 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

High-mobility group AT-hook 2 (HMGA2) is a non-histone chromatin-associated protein that belongs to the high-mobility group of proteins. As an architectural transcription factor, this protein binds to the minor groove of DNA that has AT-rich sequences and can induce changes in the chromatin structure. In addition to binding DNA and remodeling chromatin, HMGA2 can directly bind to transcription factors and stabilize protein-protein and protein-DNA interactions via the formation of enhanceosome and regulate transcription activity of several genes. HMGA2 also regulates a variety of biological processes such as the development of the embryo, cell differentiation, DNA repair, senescence, and cell cycle progression. HMGA2 is widely expressed in fetal and embryonic tissues whereas the expression in adult tissues is almost negligible. However, HMGA2 is observed to be re-expressed in a variety of human malignancies, thus indicating its role in tumorigenesis and cancer progression. Overexpression of HMGA2 is associated with cancer metastasis and poor prognosis, thus indicating that it could be a potential diagnostic and prognostic marker for cancer. BioVision’s HMGA2 (Human) ELISA kit is based on the Sandwich principle to quantitatively measure the concentration of HMGA2 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of HMGA2 in the sample or standard.


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