HMG-CoA Reductase Activity Assay Kit (Colorimetric) (ab204701)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 10 min
- Sample type: Purified protein
Overview
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Product name
HMG-CoA Reductase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Purified protein -
Assay type
Enzyme activity -
Assay time
0h 10m -
Product overview
HMG-CoA Reductase Activity Assay Kit (Colorimetric) (ab204701) is suitable for measuring activity of purified HMG-CoA reductase or for screening inhibitors/activators of HMG-CoA reductase. It is based on the consumption of NADPH by the enzyme, which can be measured by the decrease of absorbance at OD=340 nm. The limit of detection is below 0.05 mU.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K588 H mg-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric). K588-100 is the same size as the 100 test size of ab204701.
HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is the rate-controlling enzyme of the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. The enzyme is target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Atorvastatin Solution 1 x 10µl HMG-CoA 1 vial HMG-CoA Reductase 1 vial HMG-CoA Reductase Assay Buffer 1 x 20ml NADPH V 1 vial -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
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Function
This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis. -
Pathway
Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3. -
Sequence similarities
Belongs to the HMG-CoA reductase family. -
Cellular localization
Endoplasmic reticulum membrane. Peroxisome membrane. - Information by UniProt
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Alternative names
- 3 hydroxy 3 methylglutaryl CoA reductase
- 3 hydroxy 3 methylglutaryl Coenzyme A reductase
- 3 hydroxymethylglutaryl CoA reductase
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab204701 has been referenced in 5 publications.
- Abu Helal R et al. Aortic Fibrosis in Insulin-Sensitive Mice with Endothelial Cell-Specific Deletion of Ceacam1 Gene. Int J Mol Sci 23:N/A (2022). PubMed: 35457157
- Luo G et al. Discovery of an orally active VHL-recruiting PROTAC that achieves robust HMGCR degradation and potent hypolipidemic activity in vivo. Acta Pharm Sin B 11:1300-1314 (2021). PubMed: 34094835
- Waku T et al. NRF3 upregulates gene expression in SREBP2-dependent mevalonate pathway with cholesterol uptake and lipogenesis inhibition. iScience 24:103180 (2021). PubMed: 34667945
- Ghadieh HE et al. Loss of Hepatic Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Links Nonalcoholic Steatohepatitis to Atherosclerosis. Hepatol Commun 4:1591-1609 (2020). PubMed: 33163831
- Xu C et al. CSA13 inhibits colitis-associated intestinal fibrosis via a formyl peptide receptor like-1 mediated HMG-CoA reductase pathway. Sci Rep 7:16351 (2017). PubMed: 29180648