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HIV-2 Protease Activity Assay Kit (Fluorometric)

Sensitive fluorometric assay to detect HIV-2 Protease activity.
Catalog #: K845
$495.00

Product Details

Cat # +Size K845-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 330/450 nm)
Applications Detect activity of recombinant HIV-2 protease
Determine activity of purified HIV-2 protease
Features & Benefits • Fluorometric detection.
Kit Components • HIV-2 Protease Assay Buffer
• HIV-2 Protease Dilution Buffer
• HIV-2 Protease Substrate
• HIV-2 Protease (Positive Control)
• Fl. Standard (10 mM in DMSO)
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). Infection with HIV-2 virus causes immunosuppression, as well as AIDS, characterized by the same signs, symptoms and opportunistic infections as HIV-1. However, HIV-2-associated AIDS may often be associated with lower viral load levels than HIV-1. Aspartyl HIV-2 Protease (retropepsin) is an essential enzyme for the life-cycle of HIV virus. Expressed as a part of the Gag-Pol polyprotein complex in the infected cells, it is auto-catalytically released upon formation of an immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins resulting in the maturation of the virus. In HIV-infected patients the enzyme is subjected to intensive mutagenesis and mutants resistant to applied medicines are produced as a result of the selection pressure. BioVision’s HIV-2 Protease Activity assay kit utilizes the ability of active HIV-2 Protease to cleave a synthetic peptide substrate to release the free fluorophore which can be easily quantified (Ex/Em = 330/450 nm) using a fluorometer or fluorescence microplate reader. This assay is simple, rapid and can detect HIV-2 protease activity in samples and of purified enzyme.


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