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HIV-1 Protease Inhibitor Screening Kit (Fluorometric)

Market's first and only kit for screening HIV-1 Protease Inhibitors. Simple and HTP compatible.
Catalog #: K826
$530.00

Product Details

Cat # +Size K826-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 330/450 nm)
Species Reactivity All
Applications Screening/studying/characterizing inhibitors of HIV-1 Protease The simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HIV-1 Protease with a fluorometer or fluorescence microplate reader.
Features & Benefits Detect using a fluorometer or fluorescence microplate reader.
Kit Components • HIV-1 Protease Assay Buffer
• HIV-1 Protease Dilution Buffer
• HIV-1 Protease Substrate
• HIV-1 Protease
• HIV-1 Protease Inhibitor (1 mM Pepstatin A in DMSO)
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of the virus as it cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Without effective HIV-1 protease, HIV virions remain non-infectious. BioVision’s HIV-1 Protease Inhibitor Screening kit utilizes the ability of active HIV-1 protease to cleave a synthetic peptide substrate resulting in the release of free fluorophore, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a HIV-1 Protease inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HIV-1 Protease.


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