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HIV-1 Protease Activity Assay Kit (Fluorometric)

Sensitive fluorometric assay to detect HIV-1 Protease activity.
Catalog #: K825
$475.00

Product Details

Cat # +Size K825-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 330/450 nm)
Applications Detect activity of recombinant HIV-1 protease
Determine activity of purified HIV-1 protease
Features & Benefits Fluorometric detection
Kit Components HIV-1 Protease Assay Buffer
HIV-1 Protease Dilution Buffer
HIV-1 Protease Substrate
Fl. Standard (10 mM in DMSO)
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Human Immunodeficiency Virus (HIV) is the cause of the Acquired Immunodeficiency Syndrome (AIDS). HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of the virus as it cleaves newly synthesized polyproteins at the appropriate places to create the mature protein components of an infectious HIV virion. Without effective HIV1 protease, HIV virions remain non-infectious. BioVision’s HIV-1 Activity assay kit utilizes the ability of active HIV-1 protease to cleave a synthetic peptide substrate to release the free fluorophore which can be easily quantified (Ex/Em = 330/450 nm) using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect HIV-1 protease activity in samples and of purified enzyme.


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