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Histamine Assay Kit (Colorimetric)

Enzymatic kit for the detection of histamine in biological samples
Catalog #: K506
$415.00

Product Details

Cat # +Size K506-100
Size 100 assays
Detection Method Colorimetric (OD: 450 nm)
Applications Estimation of histamine in various biological samples
Features & Benefits • Simple, rapid & convenient assay
• Limit of detection: 10 µM
• Very specific, does not interact with other bioamines
Kit Components • Histamine Assay Buffer
• Histamine Enzyme Mix
• HIstamine Probe
• Histamine Standard (50 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Histamine is a biogenic amine with considerable biological relevance. As a signaling molecule histamine plays manifold roles in the body, ranging from local immune responses to neurotransmission. Histamine is also frequently encountered in food, particularly fish and fermented food products such as sauerkraut and aged cheeses. This is because some bacteria generate histamine from histidine via the enzyme histidine decarboxylase. Elevated levels of bacterial fermentation can lead to elevated histamine in raw meat and food products. Histamine levels thus can be used as an indicator of spoilage. While the body is accustomed to exposure to low levels (30-50 ppm, or mg/l) of histamine, ingestion of food containing levels higher than several hundred parts per million can lead to adverse effects. This is known as histamine poisoning. BioVision’s Histamine Assay Kit can thus be used to identify dangerous levels of histamine in various fish and meat products, as well as other fermented food and sauces/beverages. With our kit, histamine can be detected in samples at concentrations as low as 100 μM, or 10 μg/ml (10 ppm).


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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Is the kit compatible with plasma or serum sample from mouse/human ?
We have tested the kit with putrid fish and fish sauce. The amount of histamine in serum/plasma samples are very low. Thus, this kit will not work for human or mouse plasma or serum samples.