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His-tag Protein ELISA kit

A competitve ELISA kit for the quantitative measurement of Bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies
Catalog #: E4550

In stock

$350.00

Product Details

Cat # +Size E4550-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of His-Tag Protein in bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications This ELISA kit is used for quantitative measurement of His-Tag Protein in bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies
Features & Benefits • This ELISA kit is used for in vitro quantitative determination of His-Tag Protein
• Detection Range: 25.6 – 3200 ng/ml
• Sensitivity: 20 ng/ml
Kit Components • ELISA Microplate
• Standard
• HRP-conjugate
• Antibody Stock
• TMB substrate
• Stop Solution
• Sample Diluent
• Antibody Diluent
• Wash Buffer
• Plate Sealers
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

A His-tag is an amino acid motif that contains at least six consecutive histidine (His) residues, often at the N- or C-terminus of a protein. More than 50% of recombinant proteins expressed by eukaryotic or prokaryotic expression systems are tagged with His-tag. Selective, sensitive and quantitative detection of protein levels and protein-protein binding partners expressed with His-tag is therefore a valuable tool in Life Science research. BioVision’s His-Tag Protein ELISA Kit is a competitive-based ELISA that is easier, faster and more sensitive method to detect His-tag proteins expressed in both bacterial, insect and mammalian cells. This detection kit offers ready-to-use reagents, and can detect as low as 20 ng/ml of His-tag protein within 90 min.


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