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Hexokinase (Rat) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of hexokinase in rat serum, plasma, culture supernatants and other biological fluids.
Catalog #: E4600

In stock

$695.00

Product Details

Cat # +Size E4600-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Rat
• Application- quantitative measurement of hexokinase in rat serum, plasma, tissue homogenates and other biological fluids.
Detection Method Absorbance (450 nm)
Species Reactivity Rat
Applications This ELISA kit is used for quantitative measurement of hexokinase in rat serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Easy, convenient and time-saving method to measure the level of hexokinase in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Detection Range: 31.25 - 2000 pg/ml
• Sensitivity: < 18.75 pg/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10% No significant cross-reactivity or interference was observed.
Kit Components • Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km. Hexokinase deficiency leads to severe human diseases such as X-linked muscular dystrophy and a rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis. Early detection of abnormal hexokinase activity is crucial for diagnosis, prediction and treatment of the disease. BioVision’s Hexokinase ELISA kit is a sandwich ELISA assay for the quantitative measurement of Hexokinase in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Hexokinase captured from the samples.


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