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Hexokinase (HK) Inhibitor Screening Kit (Colorimetric)

Market's first and only kit for screening Hexokinase Inhibitors. Simple and HTP compatible. Screen within 30 min.
Catalog #: K828

Product Details

Cat # +Size K828-100
Size 100 assays
Detection Method Absorbance at 450 nm
Applications Screening/characterizing/studying potential inhibitors of Hexokinase.A simple and high-throughput adaptable assay kit used to colorimetrically (450 nm) screen/study/characterize potential inhibitors of Hexokinase within 30 min.
Features & Benefits Simple and High throughput adaptable
Colorimetric Detection
Screen within 30 min
Kit Components • HK Assay Buffer
• HK Substrate
• HK Coenzyme
• HK Converter
• HK Developer
• Hexokinase
• HK Inhibitor Control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Hexokinases (HK) play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km. Hexokinase deficiency leads to severe human diseases such as X-linked muscular dystrophy and a rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various tumors and is associated with metastasis. In BioVision’s Hexokinase Inhibitor Screening kit, glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. In the presence of a Hexokinase inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of absorbance. This assay kit can be used to screen/study/characterize the potential inhibitors of Hexokinase. The assay is simple, high-throughput adaptable and can be performed within 30 min.

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