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Hepcidin (Rat) ELISA Kit

A Sandwich ELISA kit for the qualitative detect Hepcidin in rat serum or plasma.
Catalog #: E4694

In stock


Product Details

Cat # +Size E4694-100
Size 96 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- rat
• Application- quantitative measurement of Hepcidin in serum, plasma, tissue homogenates and other biological fluids.
Detection Method colormetric
Species Reactivity Rat
Applications This ELISA kit is used for in vitro quantitative determination of Hepcidin in rat samples.
Features & Benefits • Sensitivity: < 9.375 pg/ml
• Detection Range: 15.625-1000 pg/ml
• No significant cross-reactivity or interference was observed
• Easy, convenient and time-saving method to measure the level of Hepcidin in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
Kit Components • Wash buffer (25X)
• SABC dilution buffer
• Sample / Standard dilution buffer
• TMB substrate (Avoid light)
• Micro ELISA Plate
• Lyophilized Standard
• Plate sealers
• Antibody dilution buffer
• Biotin- detection antibody (Concentrated)
• HRP-Streptavidin Conjugate (SABC) (Avoid light)
• Stop Solution
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Hepcidin is a liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes. BioVision’s Hepcidin ELISA kit is a sandwich ELISA assay for the quantitative measurement of Hepc in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Hepcidin captured from the samples.

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