Heparin Sepharose Column (ab286844)
Overview
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Product name
Heparin Sepharose Column -
Tested applications
Suitable for: Purificationmore details -
General notes
Heparin-beads have been widely used in affinity purification of various heparin-binding proteins or ligand, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and other ligands. ab286844 is designed for the purpose of purification of these kinds of proteins and ligands. It can also be used as a high capacity cation exchange medium. Specific proteins can be separated by using different concentrations of salt or a salt gradient. This HeparinSepharose formulation exhibits excellent binding capacity, high flow rate, no significant loss of the heparin ligand and a pH stability range of 2-10.
Heparin-Sepharose is prepared by covalently coupling heparin to epoxy-activated 6% cross-linked sepharose beads. The coupling was optimized to give a high binding capacity and could be greater than 0.4 mg of heparin-binding protein (such as thrombin) per ml of wet gel.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. The product can be stored for up to 12 months. -
Storage buffer
Constituent: 20% Ethanol -
Concentration information loading...
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab286844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Purification |
Use at an assay dependent concentration.
Purification of heparin-binding proteins, enzymes or other ligands |
Notes |
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Purification
Use at an assay dependent concentration. Purification of heparin-binding proteins, enzymes or other ligands |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab286844 has not yet been referenced specifically in any publications.