HeLa Nuclear Extract

For western blotting and HDAC assay positice controls.

1641 is available from Abcam as ab286851.
Catalog #: 1641 | abID: ab286851

Product Details

abID ab286851
Highlights • Contents- 20 µl (100 µg) or 200 µl (1 mg) of HeLa Nuclear Extract (5 mg/ml) in 20 mM Hepes (pH 7.8), 100 mM KCl, 1 mM MgCl₂, 20% Glycerol, 0.5 mM DTT, and 1 mM PMSF.
• Application- Western blot- 10-20 µg/lane; HDAC Fluorometric Assay- 10 µg/assay; HDAC Colorimetric Assay- 50 µg/assay
Storage Conditions -70°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.


Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.

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Sangwon Chung, Free fatty acid-induced histone acetyltransferase activity accelerates lipid accumulation in HepG2 cells. Nutr Res Pract, Apr 19; 31214287.
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