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Product Details
| Highlights | • Contents- 20 µl (100 µg) or 200 µl (1 mg) of HeLa Nuclear Extract (5 mg/ml) in 20 mM Hepes (pH 7.8), 100 mM KCl, 1 mM MgCl₂, 20% Glycerol, 0.5 mM DTT, and 1 mM PMSF. • Application- Western blot- 10-20 µg/lane; HDAC Fluorometric Assay- 10 µg/assay; HDAC Colorimetric Assay- 50 µg/assay |
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| Storage Conditions | -70°C |
| Shipping Conditions | Dry Ice |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Nuclear extract is prepared from HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins, transcription factors, and other nuclear proteins.
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Sangwon Chung, Free fatty acid-induced histone acetyltransferase activity accelerates lipid accumulation in HepG2 cells.
Nutr Res Pract, Apr 19; 31214287.
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