Product Details
| Cat # +Size | 6871-25 |
|---|---|
| Size | 25 μg |
| Antibody Target | H3K9/14ac |
| Alternate Name | Histone 3 |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Polyclonal antibody raised in rabbit against histone H3 acetylated at lysines 9 and 14 (H3K9/14ac), using a KLH-conjugated synthetic peptide. |
| Accession # | P68431 |
| Gene ID | 8350 |
| Appearance | Colorless liquid |
| Formulation | In PBS with 0.05% sodium azide and 0.05% ProClin 300. |
| Purification | Affinity Purified |
| Species Reactivity | Human, mouse. |
| Application | Western blot, IF, ELISA, Dot Blot, ChIP. |
| Positive Control | Western blot: HeLa cells, IF: NIH3T3 cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides. |
| Application & Usage | WB: 1:1000, Dot Blot: 1:20,000, ChIP: 1-2 µl/ChIP, IF: 1:500, ELISA: 1:100. |
| Handling | The antibody solution should be gently mixed before use. |
| Storage Conditions | -20 °C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of H3K9/14 is enriched near the promoters of active genes.
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