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H3K27me3S28p polyclonal antibody

Polyclonal Antibody to detect H3K27me3S28p in human samples.
Catalog #: 6810
$245.00

Product Details

Cat # +Size 6810-50
Size 50 μl
Antibody Target H3K27me3S28p
Alternate Name Histone H3
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen KLH-conjugated synthetic peptide of Histone H3 containing trimethylated lysine 27 and the phosphorylated serine 28.
Accession # P68431
Gene ID 8350
Appearance Colorless liquid
Formulation In PBS with 0.05% (W/V) sodium azide.
Purification Whole antiserum from rabbit
Species Reactivity Human.
Application Western blot, IF, ELISA, ChIP, Dot blot.
Positive Control Western blot: HeLa cells, IF: HeLa cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides.
Application & Usage IF: 1:200, WB: 1:250 – 1:500, ELISA: 1:100 – 1:500, Dot Blot: 1:20,000, ChIP: 1 µl/ChIP, IP: 5 µl/IP.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Phosphorylation of H3 on serine 28 is increased during mitosis.


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