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H-Cadherin (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of H-Cadherin

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5123
$725.00

Product Details

Cat # +Size E5123-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of H-Cadherin in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Sensitivity: 0.094 ng/ml
● Detection range: 0.156-10 ng/ml
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Highly sensitive and specific
● Recovery Rate: 85-105% for serum, 90-99% for EDTA plasma and 87-103% for heparin plasma
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (10 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

H-cadherin also known as Cadherin-13, CDH13 or T-cadherin, is a unique member of cadherin superfamily because it lacks the transmembrane and cytoplasmic domains and is anchored to the cell’s membrane through the GPI anchor. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Thus, cadherins contributes to the sorting of heterogeneous cell types. The cadherin family is essential in maintaining the cell-cell contact and regulate cytoskeletal complexes. H-cadherin is a GPI-anchored member of cadherin superfamily, which lacks a direct contact with cytoskeleton and therefore is not involved in cell–cell adhesion. It is involved in low density lipoproteins hormone-like effects on Ca2+ mobilization and increased cell migration as well as phenotype changes. H-Cadherin act as an independent prognostic biomarker in patients with bladder TCC. BioVision’s H-Cadherin (Human) ELISA Kit is used for the quantitative detection of H-Cadherin in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of H-Cadherin in the sample.


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