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GSTA4, human recombinant

Involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds
Catalog #: 7800
$325.00

Product Details

Cat # +Size 7800-100
Size 100 μg
Alternate Name Glutathione S-transferase alpha 4, GSTA4-4, GTA4
Gene Symbol GSTA4
Gene ID 2941
Accession # O15217
Source E. coli.
Appearance Liquid
Physical Form Description 1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 20% glycerol, 2 mM DTT and 100 mM NaCl.
Molecular Weight 28.3 kDa (246 aa, 1-222 aa + His Tag)
Purity by SDS-PAGE ≥95%
Biological Activity Specific activity > 0.9 units/mg, one unit is defined as the amount of enzyme that conjugate 1.0 µmole of 1-chloro-2, 4- dinitrobenzene (CDNB), with reduced glutathione per minute pH 6.5 at 25ºC.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQD GNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDD QQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTV KLSNIPTIKR FLEPGSKKKP PP
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

GSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows very high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is highly effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, an important product of peroxidative degradation of arachidonic acid and a commonly used biomarker for oxidative damage in tissue. This enzyme is expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. Recombinant human GSTA4 protein, fused to His-tag at N-terminus, was expressed in E.Coli and purified by using conventional chromatography techniques.


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