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Granzyme B (human) ELISA Kit

Sensitive, Colorimetric Assay

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: K4279

Product Details

Cat # +Size K4279-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity human
Applications This ELISA kit is used for quantitative protein detection, establishing normal range, validation of antibody array results
Features & Benefits • Easy, convenient and time-saving method to assay for human Granzume B.
• Detection Range:15.6 pg/ml – 1000 pg/ml. Sensitivity: < 10 pg/ml.
• This ELISA kit shows no cross-reactivity with other relevant proteins.
• Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.
Kit Components • 96 wells coated with anti-human monoclonal Granzyme B antibody, 1 Microplate
• Lyophilized recombinant human Granzyme B standard (10 ng/vial)
• Biotinylated anti- human Granzyme B antibody
• Avidin-Biotin-Peroxidase Complex (ABC)
• Sample diluent buffer
• Antibody diluent buffer
• ABC diluent buffer
• TMB color developing agent (Colorless)
• TMB stop solution
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Granzyme B is a serine protease expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells and functions in cell-mediated immunity. BioVision’s human Granzyme B ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human Granzyme B is coated on a 96-well plate. Standards and test samples are added to the wells and Granzyme B present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Granzyme B is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Granzyme B captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 10 pg/ml.

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