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GOT1, human recombinant

Plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles.
Catalog #: 7808
$305.00

Product Details

Cat # +Size 7808-100
Size 100 μg
Alternate Name Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18
Gene Symbol GOT1
Gene ID 2805
Accession # P17174
Source E. coli.
Appearance Liquid
Physical Form Description 0.5 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 100 mM NaCl and 1 mM DTT.
Molecular Weight 48.4 kDa (433 aa, 1-413 aa + His Tag)
Purity by SDS-PAGE ≥95%
Biological Activity Specific activity is > 13 unit/mg, in which one unit will convert 1.0 µmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25ºC.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SV
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.


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