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GnRH ELISA Kit

A Competitive ELISA kit for in vitro quantitative determination of GnRH concentrations in serum, plasma and other biological fluids.

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5099
$695.00

Product Details

Cat # +Size E5099-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity All
Applications The ELISA kit is used for in vitro quantitative determination of GnRH concentrations in serum, plasma and other biological fluids.
Features & Benefits ● Detection Range: 15.63-1000 pg/ml
● Sensitivity: 9.38 pg/ml
● Highly sensitive and specific
Kit Components ● Micro ELISA Plate
● Stop Solution
● Plate Sealer
● Standard
● Biotinylated Detection Ab (100x)
● HRP Conjugate (100x)
● Standard & Sample Diluent
● Biotinylated Detection Antibody Diluent
● HRP Conjugate Diluent
● Wash Buffer (25x)
● Substrate Reagent
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Gonadotropin-releasing hormone (GnRH) is produced and secreted by specialized nerve cells in the hypothalamus of the brain. It is released into tiny blood vessels that carry this hormone from the brain to the pituitary gland. GnRH is a releasing hormone responsible for the release of follicle-stimulating hormone and luteinizing hormone from the anterior pituitary. These hormones are released into the general circulation and act on the testes and ovaries to initiate and maintain their reproductive functions. Treatment with gonadotropin-releasing hormone agonists of uterine myoma, endometriosis and some hormone-dependent cancers, such as breast, ovarian, endometrial and prostate cancer, also seems to have a beneficial effect. BioVision’s GnRH ELISA Kit is used to quantitatively measure GnRH in Serum, plasma and other biological fluids. The kit is based on the Competitive ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with GnRH. During the reaction, GnRH in samples or Standard competes with a fixed amount of GnRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GnRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GnRH in the samples is calculated by comparing the OD of the samples to the standard curve.


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