Product Details
Cat # +Size | K4772-100 |
---|---|
Size | 100 assays |
Detection Method | Absorbance (450 nm) |
Species Reactivity | human |
Applications | This ELISA kit is used for quantitative protein detection, establishing normal range, validation of antibody array results |
Features & Benefits | • Easy, convenient and time-saving method to assay GM-CSF. • The minimum detectable concentration of GM-CSF is less than 2 pg/ml. • This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- ?, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 ?, IL-1?, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1?, MIP-1?, MIP-2, MIP-3?, MIP-3?, PF-4, P-Selectin, SCF, SDF-1?, TARC, TCA-3, TECK, TIMP-1, TNF-?, TNF RI, TNF RII, TPO, VCAM-1, VEGF. • Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed. |
Kit Components | • GM-CSF Microplate coated with anti-human GM-CSF, 96 wells • Wash Buffer Concentrate (20x) • Standards (recombinant human GM-CSF) • Assay Diluent for Standard/Sample (serum/plasma/ cell culture medium/Urine) diluent (5x con.) • Detection Antibody GM-CSF, biotinylated anti-human GM-CSF (each vial enough for half plate) • HRP-Streptavidin Concentrate, 200x concentrated • TMB One-Step Substrate Reagent 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution, 0.2 M sulfuric acid |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |
Details
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Ensure the plate reader is set at the correct wavelength recommended on the protocol