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Glyoxalase II Activity Kit (Colorimetric)

The most sensitive kit in the market to detect Glyoxalase II activity
Catalog #: K460
$450.00

Product Details

Cat # +Size K460-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Sample type- tissue and cell culture lysate, red blood cells
• Species reactivity- Mammalian
• Application- Measurement of Glyoxalase II as low as 0.6 mU/ml.
Detection Method Colorimetric (OD 450 nm)
Species Reactivity Mammalian
Applications Measurmente of Glyoxalase II activity in biological samples
Features & Benefits • Simple procedure; takes ~ 40 minutes
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Glyoxalase II
Kit Components • GloII Assay Buffer
• Enzyme Mix (Lyophilized)
• GloII Substrate (Lyophilized)
• GloII Probe (Lyophilized)
• GloII Positive Control
• D-Lactate Standard (100 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glyoxalase II (GloII, hydroxyacylglutathione hydrolase, EC 3.1.2.6) is one of the two enzymes in the Glyoxalase system that is ubiquitously expressed in mammalian, plants and bacteria. The Glyoxalase system is a biological pathway that detoxifies cells by metabolizing α-ketoaldehydes such as methylglyoxal, a cytotoxic byproduct of lipids and glucose metabolism. GloII fulfills the terminal step in the glyoxalase system: It catalyzes the hydrolysis of S-D-lactoyl-glutathione (SLG) to reduced glutathione and D-lactic acid. The alteration of GloII activity in cell growth cycle, cell differentiation, phagocyte activation and diabetes mellitus development of implies this enzyme plays a wide-ranging and important role in cell function and disease progression. Research areas of current interest in Glo-II include diabetes, and cancer therapy. Biovision’s Glyoxalase II Activity Kit utilizes the ability of an active Glo-II to cleave a substrate while producing D-lactate. The produced D-Lactate reacts with the chromophore generating a stable signal that can be easily quantified at 450 nm using a microplate reader. Our assay kit is simple, sensitive and can detect as low as 0.6 mU/ml of Glo-II activity in biological samples


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