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New

Glycolic Acid Assay Kit (Fluorometric)

for measuring GA in serum
Catalog #: K2022
$525.00

Product Details

Cat # +Size K2022-100
Size 100 assays
Kit Summary BioVision’s Glycolic Acid assay kit is a robust, one step plate based assay for the measurement of GA in serum. The GA Detection Reagent metabolizes GA leading to the formation of an intermediate. The intermediate in the presence of GA enzyme mix then converts the non-fluorescent GA probe to a fluorescent product, which is measured at Ex/Em = 535/587 nm. This kit can quantify as low as 30 pmol of GA.
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity All
Applications Quantification of Glycolic acid
Features & Benefits • One step plate based assay for the measurement of GA in serum
• Robust
Kit Components GA Probe
GA Detection Reagent
GA Standard
GA Assay Buffer
GA Enzyme Mix
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glycolic Acid (GA), also known as Hydroxyl Acetic Acid is the smallest alpha hydroxy acid. Although it is used in several skin care products intended for dermal application in small doses, its acute dose can lead to skin and eye irritation. It is nephrotoxic if swallowed and its toxicity is due to its conversion to oxalic acid, which then precipitates with calcium to form crystals leading to renal tissue injury. GA is also a major downstream metabolite of ethylene glycol metabolism, and a concentration higher than 8 mM in serum indicates the need for hemodialysis. Normally serum GA levels lie in the very low micromolar range (roughly 2 µM). BioVision’s Glycolic Acid assay kit is a robust, one step plate based assay for the measurement of GA in serum. The GA Detection Reagent metabolizes GA leading to the formation of an intermediate. The intermediate in the presence of GA enzyme mix then converts the non-fluorescent GA probe to a fluorescent product, which is measured at Ex/Em = 535/587 nm. This kit can quantify as low as 30 pmol of GA.


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