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Glycogen Synthase Kinase (GSK) (Rat) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Glycogen Synthase Kinase in rat serum, plasma, culture supernatants and other biological fluids.
Catalog #: E4610

In stock

$695.00

Product Details

Cat # +Size E4610-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Rat
• Application- quantitative measurement of Glycogen Synthase Kinase in rat serum, plasma, tissue homogenates and other biological fluids.
Detection Method Absorbance (450 nm)
Species Reactivity Rat
Applications This ELISA kit is used for quantitative measurement of Glycogen Synthase Kinase in rat serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Easy, convenient and time-saving method to measure the level of Glycogen Synthase Kinase in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Detection Range: 0.313 - 20 ng/ml
• Sensitivity: < 0.188 ng/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
• No significant cross-reactivity or interference was observed.
Kit Components • Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glycogen synthase kinase (GSK) is a serine/threonine protein kinase that has been implicated in the regulation of cell fate and in the Wnt signaling pathway. Glycogen synthase kinase is an enzyme including GSK1, GSK2, and GSK3. GSK-3 plays an important role in the PI3 kinase and Akt mediated cell survival pathways, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β. GSK-3 has also been implicated in alzheimer’s disease. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3. BioVision’s GSK ELISA kit is a sandwich ELISA assay for the quantitative measurement of GSK in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of GSK captured from the samples.


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