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Glycogen Phosphorylase Colorimetric Assay Kit

for detecting glycogen phosphorylase activity
Catalog #: K179
$485.00

Product Details

Cat # +Size K179-100
Size 100 assays
Kit Summary BioVision’s Glycogen Phosphorylase Colorimetric Assay Kit is rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity. The kit can detect as low as 10 mU in a variety of sample types.
Detection Method Absorbance, OD 450 nm
Species Reactivity All
Applications Analysis of glycogen phosphorylase kinetics, inhibition or activation, Measurement of glycogen phosphorylase activity in various tissues or cells
Features & Benefits • Sensitive
• Rapid
• Convenient tool for detecting glycogen phosphorylase activity
• The kit can detect as low as 10 mU in a variety of sample types
Kit Components • Glycogen
• Assay Buffer
• G1P Standard
• Substrate Mix
• Glycogen Phosphorylase
• Enzyme Mix
• Developer
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glycogen phosphorylase (EC 2.4.1.1) catalyzes the rate-limiting step in glycogenolysis using glycogen and inorganic phosphate to produce glucose-1-phosphate (G1P). In mammals, glycogen phosphorylase is abundant in muscle, liver, and brain tissues. There are two forms of glycogen phosphorylase, namely glycogen phosphorylase a and b forms. Glycogen phosphorylase a is the highly active form whereas glycogen phosphorylase b has only limited activity. Glycogen phosphorylase is clinically significant enzyme as its mutations are associated with different glycogen storage diseases in muscle and liver. In addition, this enzyme has been suggested as a biomarker for gastric cancer and its inhibition has been tested in treating type 2 diabetes. BioVision’s Glycogen Phosphorylase Activity Assay Kit is based on the detection of G1P by a set of enzymatic reactions to generate a colored product with a strong absorbance at 450 nm. The OD 450 nm signal is directly proportional to the glycogen phosphorylase activity. BioVision’s Glycogen Phosphorylase Colorimetric Assay Kit is rapid, sensitive and convenient tool for detecting glycogen phosphorylase activity. The kit can detect as low as 10 mU in a variety of sample types.


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