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GluTracker™ Glucose Uptake Assay Kit (Cell-Based)

Non-radioactive method to image and accurately measure glucose uptake via FACS
Catalog #: K681
$875.00

Product Details

Cat # +Size K681-50
Size 50 assays
Detection Method FACS (488 nm excitation laser) and fluorescent microscope
Applications Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens and nutrients, etc.
Dual-staining of glucose transporters and glucose uptake
Analysis of glucose metabolism and cell signaling in various cell types
Screening of anti-diabetic compounds
Features & Benefits • Easy-to-use
• Non-radioactive
• Image and accurately measure glucose uptake in cultured cells in response to insulin, growth factors etc.
Kit Components • Analysis Buffer (50X)• GluTracker Reagent (100X)• GluTracker Enhancer• Phloretin (100X)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glucose is a ubiquitous energy source in most organisms and plays a pivotal role in cellular metabolism and homeostasis. Cancer cells exhibit increased glucose uptake to support their high proliferation rate. At BioVision, we have developed a fluorescent glucose analog, which just like glucose can be taken up by cells through glucose transporters. However, this glucose analog cannot be fully utilized in glycolysis because of its modification and thus accumulates inside the cells. Fluorescence generated by this fluorescent glucose analog is proportional to the glucose uptake by the cells and can be used to measure glucose uptake using fluorescent microscopy and flow cytometry. To validate the assay, the kit includes phloretin, a natural phenol that inhibits glucose uptake. This easy-to-use, non-radioactive kit allows imaging and accurate measurement of glucose uptake in cultured cells in response to insulin, growth factors etc.


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Can we distinguish the uptake between Glut 1 and Glut 4 transporters?
The uptake is not specific for any particular GLUT transporter. Certain cells express more of one GLUT transporter compared to tohers and the relative contribution to uptake will depend on this. If specific inhibitor is used for one glut transporter, then the uptake by the others can be seen.
Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.