Product Details
| Cat # +Size | E4625-100 |
|---|---|
| Size | 100 assays |
| Kit Summary | • Detection method- Absorbance (450 nm) • Species reactivity- Rat • Application- quantitative measurement of Glutathione Reductase in rat serum, plasma, tissue homogenates and other biological fluids. |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Rat |
| Applications | Quantitative measurement of Glutathione Reductase in rat serum, plasma, tissue homogenates and other biological fluids. |
| Features & Benefits | • Easy, convenient and time-saving method to measure the level of Glutathione Reductase in serum, plasma, tissue homogenates, culture supernatants and other biological fluids. • Detection Range: 0.312 - 20 ng/ml • Sensitivity: < 0.188 ng/ml • Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10% No significant cross-reactivity or interference was observed. |
| Kit Components | • Micro ELISA Plate • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Glutathione reductase (GR) is a member of pyridine nucleotide-disulfide oxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG). In most eukaryotic cells, GR maintains the ratio of [GSH]/[GSSG] elevated, and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis. BioVision’s Glutathione Reductase ELISA kit is a sandwich ELISA assay for the quantitative measurement of GR in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of GR captured from the samples.
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