Product Details
| abID | ab83461 |
|---|---|
| Cat # +Size | K761-200 |
| Size | 200 assays |
| Kit Summary | BioVision’s Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. |
| Detection Method | Absorbance (405 nm) |
| Species Reactivity | Mammalian |
| Applications | The assay can detect 0.1 – 40 mU/ml GR in various samples. |
| Features & Benefits | • Simple procedure; takes ~ less than 40 minutes • Fast and convenient |
| Kit Components | • GR Assay Buffer • 3% H₂O₂ • Catalase • TNB Standard (2.5 µmol) • DTNB • NADPH-GNERAT™ • GSSG • GR Positive Control (10 mU) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. BioVision’s Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5′-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1 – 40 mU/ml GR in various samples.
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What is the source of the enzyme in the standard?
We use a yeast Glutathione Reductase.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Samir Ali Abd El-Kaream. Biochemical and biophysical study of chemopreventive and chemotherapeutic anti-tumor potential of some Egyptian plant extracts. Biochem Biophys Rep. Apr 2019. 31016248
Jiao Jiao Zhang, et al., (2018) MicroRNA-7450 regulates non-thermal plasma-induced chicken Sertoli cell apoptosis via adenosine monophosphate-activated protein kinase activation, Scientific Reports, Jun. 2018, 29884805
Parrillo, Lucia et al. (2017) Olive mill wastewater-enriched diet positively affects growth, oxidative and immune status and intestinal microbiota in the crayfish, Astacus leptodactylus, Aquaculture. 2017 Apr;473: 161-168
Seki, Keisuke et al. (2016) Varying butyric acid amounts induce different stress- and cell death-related signals in nerve growth factor-treated PC12 cells: implications in neuropathic pain absence during periodontal disease progression, Apoptosis. 2016 Jun;21(6):699-707.
Horta et al., Organ-coordinated response of early post-germination mahogany seedlings to drought. Tree Physiol, Apr 2014; 10.1093/treephys/tpu017.
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