Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

New

Glutamine Synthetase Activity Assay Kit (Colorimetric)

For measuring GS activity in biological samples
Catalog #: K2056
$495.00

Product Details

Cat # +Size K2056-100
Size 100 assays
Kit Summary Glutamine Synthetase Activity Assay Kit is a simple, plate-based colorimetric assay for measuring GS activity in biological samples. In this assay, GS hydrolyzes Glutamate to Glutamine and ADP. ADP in the subsequent enzymatic reaction, in presence of ADP Converter, ADP Developer Mix and ADP Probe forms a colorimetric product that is measured at OD 570 nm. The assay is simple, sensitive and can detect as low as 10 µU of GS in biological samples.
Detection Method Absorbance at 570 nm
Species Reactivity Mammalian
Applications Measurement of Glutamine Synthetase activity in tissue and cell lysate
Features & Benefits • A simple, plate-based colorimetric assay for measuring GS activity in biological samples.
• The assay is simple, sensitive and can detect as low as 10 µU of GS in biological samples.
Kit Components • GS Assay Buffer
• Glutamine Synthetase
• Glutamate
• ATP
• ADP Probe
• ADP Converter
• ADP Developer Mix
• ADP Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glutamine Synthetase (GS, Glutamate-Ammonia Ligase; EC 6.3.1.2) is a mitochondrial enzyme that catalyzes the ATP-dependent ligation of glutamate and ammonia to glutamine. GS is ubiquitously expressed in human tissues and plays a critical role in detoxifying ammonia and regulating the concentration of glutamate or glutamine. It is also a key enzyme in nitrogen metabolism and a principal source for protein and nucleic acid synthesis. Additionally, GS activity has been linked to several neurological disorders such as Alzheimer’s disease, schizophrenia, hepatic encephalopathy and epilepsy. Furthermore, two mutations of human GS (R324C and R341C) have been linked to congenital glutamine deficiency with severe brain malformations resulting in multi-organ failure and neonatal death. BioVision’s Glutamine Synthetase Activity Assay Kit is a simple, plate-based colorimetric assay for measuring GS activity in biological samples. In this assay, GS hydrolyzes Glutamate to Glutamine and ADP. ADP in the subsequent enzymatic reaction, in presence of ADP Converter, ADP Developer Mix and ADP Probe forms a colorimetric product that is measured at OD 570 nm. The assay is simple, sensitive and can detect as low as 10 µU of GS in biological samples.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.