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Glutamate Dehydrogenase Inhibitor Screening Kit (Fluorometric)

for high-throughput screening of GDH inhibitors
Catalog #: K185
$515.00

Product Details

Cat # +Size K185-100
Size 100 assays
Kit Summary In BioVision’s Glutamate Dehydrogenase inhibitor screening Kit, Glutamate is deaminated by GDH producing NADH, which converts a non-fluorescent probe to a fluorescent product in the presence of enzyme mix that is detected fluorometrically at Ex/Em = 535/587 nm. The kit provides a rapid, simple, sensitive plate based test, which is also suitable for high-throughput screening of GDH inhibitors.
Detection Method Fluorescence at Ex/Em = 535/587 nm
Species Reactivity Mammalian
Applications Screening of potential inhibitors of Glutamate Dehydrogenase
Features & Benefits • Simple
• Sensitive plate based test, which is also suitable for high-throughput screening of GDH inhibitors
• Rapid
Kit Components • GDH Inhibitor
• GDH Enzyme
• GDH Probe
• GDH Assay Buffer
• GDH Developer
• GDH Substrate
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glutamate dehydrogenase (GDH) (1.4.1.2) is a hexameric enzyme that catalyzes the reversible conversion of glutamate to α-ketoglutarate and ammonia while reducing NAD+ to NADH. Glutamate can be converted to α-ketoglutarate by GDH and then fluxed into the TCA cycle where it can be used to support oxidative phosphorylation, production of lipids, or to replenish key intermediates such as oxaloacetate. It is a key enzyme in the glutamine metabolic pathway. Overexpression of GDH has been shown to promote cell proliferation, migration and invasion in several tumors such as colorectal cancer. The increased reliance on glutamine metabolism makes GDH a target for therapeutic intervention in cancer. GDH inhibitors have been shown to be toxic for cancer cells in vitro. In BioVision’s Glutamate Dehydrogenase inhibitor screening Kit, Glutamate is deaminated by GDH producing NADH, which converts a non-fluorescent probe to a fluorescent product in the presence of enzyme mix that is detected fluorometrically at Ex/Em = 535/587 nm. The kit provides a rapid, simple, sensitive plate based test, which is also suitable for high-throughput screening of GDH inhibitors.


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