Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Glutamate Dehydrogenase Activity Colorimetric Assay Kit

based on 4 citations in multiple journalsGlutamate Dehydrogenase Activity Colorimetric Assay Kit44.1 4
Catalog #: K729
$365.00

Product Details

Cat # +Size K729-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian
Applications This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.
Features & Benefits • Simple procedure
• Fast and convenient
• Sensitive assays for measuring GDH activity in various biological samples and the kit is also suitable for high throughput analyses.
Kit Components • GDH Assay Buffer
• Glutamate (2 M)
• GDH Developer
• GDH Positive Control
• NADH (0.5 μmol)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to α-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes. BioVision’s GDH Assay Kit provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.
What is the source of the positive control?
The glutamate dehydrogenase enzyme is isolated from bovine liver.
How to homogenize the cells without denaturing the enzyme?
"Typically we recommend using a glass Dounce homogenizer 9 http://www.biovision.com/dounce-tissue-homogenizer-3034.html) for the best results. Cells can be mildly sonicated on ice (10-20 pulses, 50% intensity)."
Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
For the K729-100, I noticed that you do not suggest a Sample Background Control for each Sample. Do you expect that the NADH produced in the Sample will not interfere and produce high Background for this kit? If I want to include a Sample Background Control, do you recommend running a reaction well without the GDH Developer?
The way the reaction works for the kit is such that we provide excess glutamate and coenzyme NAD to favor the GDH reaction. The NADH produced as a result will be in significant amounts as compared to the Background NADH that is present in the Sample. Therefore, interference from background NADH is considered negligible in this case. However, if you want, you can also include a Background Control (without the glutamate) to test the Background OD of your Samples. If it is high, you can subtract that from the Sample OD.
Liu, F. et al., (2017) MicroRNA-200c exacerbates the ischemia/reperfusion injury of heart through targeting the glutaminase (GLS)-mediated glutamine metabolism, European Review for Medical and Pharmacological Sciences, 2017, 21: 3282-3289
Yuan et al., Glutamine promotes ovarian cancer cell proliferation through the mTOR/S6 pathway. Endocr. Relat. Cancer, Jul 2015; 22: 577 - 591.
Mansilla et al., Nitrogen Absorbed from the Large Intestine Increases Whole-Body Nitrogen Retention in Pigs Fed a Diet Deficient in Dispensable Amino Acid Nitrogen. J. Nutr., Jun 2015; 145: 1163 - 1169.
Jeong et al., SIRT4 Protein Suppresses Tumor Formation in Genetic Models of Myc-induced B Cell Lymphoma. J. Biol. Chem., Feb 2014; 289: 4135 - 4144.
Kazuhiko Maeda et al., Identification and Characterization of Porphyromonas gingivalis Client Proteins That Bind to Streptococcus oralis Glyceraldehyde-3-Phosphate Dehydrogenase. Infect. Immun., Mar 2013; 81: 753 – 763.
For more citations of this product click here