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Glutamate Carboxypeptidase II Inhibitor Screening Kit (Fluorometric)

Convenient assay for screening potential GCPII inhibitors
Catalog #: K440

Product Details

Cat # +Size K440-100
Size 100 assays
Detection Method Fluorometric (Ex/Em= 535/587 nm)
Species Reactivity All
Applications Screening/characterizing Glutamate Carboxypeptidase II inhibitors
Features & Benefits •Includes Inhibitor Control (2-PMPA)
•Simple and reliable test to screen GCPII inhibitors/activators
•High-throughput compatible"
Kit Components •Human GCPII, 2-PMPA (10 mM)
•GCPII Assay Buffer
•GCPII Enzyme Mix
•PicoProbeTM (in DMSO)
•GCPII Developer
• GCPII Substrate
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Glutamate Carboxypeptidase II [GCPII, EC; N-acetylated-alpha-linked acidic dipeptidase (NAALADase), prostate-specific membrane antigen (PSMA) or folate hydrolase (FOLH1)] is a multifunctional zinc-metallopeptidase. Within the last decade, GCPII has been studied as a promising pharmacological target for the treatment of various pathological disorders and diseases and tremendous efforts have been made in order to discover potent GCPII inhibitors. NAALADase, the brain isozyme, plays an important role in Glutamate biosynthesis and could be used for treating neuronal damage caused by excess glutamate in brain. For example, inhibition of GCPII has shown neuroprotective characteristics in animal models suffering several acute and chronic conditions (stroke, neuropathic and inflammatory pain, schizophrenia, depression or amyotrophic lateral sclerosis). In males, PSMA is overexpressed in patients suffering prostate cancer and thus, it is used as a diagnostic marker for this disease. BioVision’s Glutamate Carboxypeptidase II Inhibitor Screening Kit is an in vitro tool in order to screen potential human GCPII inhibitors. The provided substrate is transaminated in the presence of GCPII producing glutamate. The detection system is based on an enzymatic reaction in which a fluorogenic probe is reduced generating a stable signal. In the presence of 2-PMPA, a potent GCPII Inhibitor, the enzymatic activity is arrested, thus generating lower fluorometric signal. The assay kit is a simple plate-based format and provides a rapid and reliable test for high-throughput screening of GCPII inhibitors.

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