Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Glucosylceramidase Activity Assay Kit (Fluorometric)

To monitor Glucosylceramidase activity
Catalog #: K2003
$495.00

Product Details

Cat # +Size K2003-100
Size 100 assays
Kit Summary BioVision’s Glucosylceramidase Activity Assay Kit provides a simple, rapid way to monitor Glucosylceramidase activity in a wide variety of biological samples. In this kit, Glucosylceramidase cleaves a specific synthetic substrate and releases a fluorophore, which can be easily quantified (Ex/Em=360/445 nm). The assay is specific, sensitive and can detect as low as 0.2 µU of Glucosylceramidase activity in variety of samples.
Detection Method Fluoroescence (Ex/Em = 360/445 nm)
Species Reactivity All
Applications • Screening for Gaucher Disease
• Measurement of Glucosylceramidase activity in various tissues/cells
Features & Benefits • Specific
• It can detect as low as 0.2 µU of Glucosylceramidase activity in variety of samples
• Sensitive
Kit Components • 4-Methylumbelliferone Standard
• Glucosylceramidase Stop Buffer
• Glucosylceramidase Positive Control
• Glucosylceramidase Assay Buffer
• Glucosylceramidase Substrate
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Lysosomal glucosylceramidase beta also known as acid beta-glucosidase (E.C. 3.2.1.45) is a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide (GC). The enzyme plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through the production of ceramides, it participates in the PKC-activated salvage pathway of ceramide formation. Glucosylceramidase also plays a role in cholesterol metabolism. It may catalyze the glucosylation of cholesterol through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol. Deficiency of Glucosylceramidase results in the accumulation of GC in lysosomes. This leads to Gaucher disease, an inherited disorder that affects many organs and tissues. BioVision’s Glucosylceramidase Activity Assay Kit provides a simple, rapid way to monitor Glucosylceramidase activity in a wide variety of biological samples. In this kit, Glucosylceramidase cleaves a specific synthetic substrate and releases a fluorophore, which can be easily quantified (Ex/Em=360/445 nm). The assay is specific, sensitive and can detect as low as 0.2 µU of Glucosylceramidase activity in variety of samples.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.