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Glucose Colorimetric/Fluorometric Assay Kit

based on 41 citations in multiple journalsGlucose Colorimetric/Fluorometric Assay Kit415 5
Sensitive Assay, HTS
Catalog #: K606

In stock

$395.00

Product Details

Cat # +Size K606-100
Size 100 assays
Detection Method Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications The kit detects 1-10000 µM glucose samples.
Features & Benefits • Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit provides all necessary buffers and reagents for a accurate assay of glucose in various mammalian samples.
Kit Components • Glucose Assay Buffer
• Glucose Probe (in DMSO)
• Glucose Enzyme Mix (lyophilized)
• Glucose Standard (100 nmol/ml)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glucose (C₆H₁₂O₆; FW: 180.16) is a very important fuel source to generate universal energy molecules ATP. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes. The Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). Glucose Enzyme Mix specifically oxidizes glucose to generate a product which reacts with a dye to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the glucose amount. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit detects 1-10000 µM glucose samples.


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Will the phenol red in the media affect the assay readout?
Very low amounts of media are used for each sample. This will generate a very low background at the best. Please use only media as a background control and subtract this reading from all sample readings to accommodate for the phenol red.
Why does the final color development starts as pink, then goes to brown then disappears?
This is a very common phenomenon observed with use of the oxired probe and is caused due to excessive analyte concentration in the samples. Therefore, if your glucose samples are too concentrated, you may see this. Please dilute your samples with the assay buffer before reanalyzing.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Can this kit be used with samples like bacteria, plants, drosophila etc?
We have optimized the kit with mammalian samples. However, theoretically these kits should work with samples from multiple species/sources. Since the optimal conditions depend on the sample type, the protocol has to be be adapted to fit the samples for efficient results. Please refer to this kit's citations to see what kind of samples have been used with this kit other than mammalian samples.
Can frozen tissue be used with this kit?
Although not ideal, flash-frozen tissue or cells stored at -80C can be used. It is important to remember that results may vary between fresh and frozen samples.
Is it possible to use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Why are standard curve values lower than those shown on the datasheet?
There are multiple factors which influence the signals like the incubation times, room temperature, handling etc. In general, to increase the value of the standards, the incubation time can be increased. As long as the standard curve is linear, it should be fine to use, since all of your samples will also be measured under the same conditions on this curve.
How are samples normalized against protein concentration?
A protein quantitation assay can be used on the supernatants from cell/tissue lysates or with any other liquid sample in the assay buffer.
Can individual components of this kit be purchased?
Yes, any of the kit's components can be purchased without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Does high sucrose concentration in the sample affect this assay's performance?
Yes, we have data comparing glucose in presence and absence of high sucrose (0.25M and 1M-effect almost identical) showing that the sucrose conc affects the assay. Therefore, this kit is not recommended to be used with samples.
What are the difference between the K606, K686 and K688 assay kit?
There are many differences between these 3 kits although they all measure the sample glucose concentration. A few of these differences which might make your decision of making a choice easier are:K606-100:Applicable for both colorimetric and fluorimetric detection.Requires filters for 570 nm and Ex/Em: 535/587 nm.Detetion limit of 1-10000 uM glucoseUtilizes the oxired probeK686-100Applicable only for colorimetric detection.Requires filters for 450 nm.Detetion limit of 20 uM to 10 mM glucoseDoes not utilize Oxired or Picoprobe (TM)Ideal for samples having reducing substancesK688-100Applicable only for fluorimetric detection.Requires filters for Ex/Em: 535/587 nm.Detetion limit of < 0.5 uM glucoseUtilizes the Picoprobe (TM)
Is it essential to make a standard curve for every expt, or is one curve/kit enough?
Yes, we strongly recommend you measure the standards every time you do an experiment. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.
Singh, Vikash et al. (2017) Salmonella Co-opts Host Cell Chaperone-mediated Autophagy for Intracellular Growth, J Biol Chem. 2017 Feb 3;292(5):1847-1864.
Yasuma, Taro et al. (2016) Amelioration of Diabetes by Protein S Diabetes. 2016 Jul;65(7):1940-51.
Gong Y et al., (2017) Blockage of glycolysis by targeting PFKFB3 alleviates sepsis-related acute lung injury via suppressing inflammation and apoptosis of alveolar epithelial cells, Biochem Biophys Res Commun., 2017, May 30: epub
Li, Xiaogang et al. (2016) Sensitization of hepatocellular carcinoma cells to irradiation by miR‑34a through targeting lactate dehydrogenase‑A, Mol Med Rep. 2016 Apr;13(4):3661-7.
Vi, Lei et al. (2016) Thrombospondin-4 ablation reduces macrophage recruitment in adipose tissue and neointima and suppresses injury-induced restenosis in mice, Atherosclerosis. 2016 Apr;247:70-7.
Cai, L. et al. (2016) Adjuvant therapies using normobaric oxygen with hypothermia or ethanol for reducing hyperglycolysis in thromboembolic cerebral ischemia, Neuroscience. 2016 Mar 24;318:45-57.
For more citations of this product click here