Glucose Colorimetric Assay Kit II

For samples having reducing substances
Catalog #: K686 | abID: ab102517

Product Details

abID ab102517
Cat # +Size K686-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian
Applications The kit can detect glucose concentrations in the range of 20µM – 10mM
Features & Benefits • Simple procedure; takes ~ 30 minutes to complete the assay
• Fast and convenient
Kit Components • Glucose Assay Buffer
• Glucose Substrate Mix
• Glucose Enzyme Mix
• Glucose Standard (100 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Glucose is an important fuel source to generate the universal energy molecule ATP. Serum glucose level is a key diagnostic parameter for many metabolic disorders. BioVision’s Glucose Assay Kit II provides direct measurement of glucose in various biological samples (e.g., serum, plasma, other body fluids, food, growth media, etc.). In this assay, glucose is specifically oxidized to generate a product which reacts with a dye to generate color (λ = 450 nm) whose intensity is proportional to glucose concentration. The method is rapid, simple, sensitive, and suitable for high throughput. This assay is particularly suitable for serum and urine samples since it is unaffected by reducing substances which can interfere with other suppliers offering oxidase-based kits. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit can detect glucose concentrations in the range of 20µM – 10mM.

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Can I use this kit with tissue samples? If yes, can you provide a protocol for the sample preparation?
Yes, this kit can be used with tissue samples. Weigh 20-50 mg tissue, add 100-200 µl of cold assay buffer. Homogenize using a dounce homogenizer or by mild sonication on ice. Once the tissue is completely homogenized, centrifuge the homogenate at 10,000 g for 5 min. Take the supernatant and use it for the assay. We recommend deproteinizing the supernatant using a 10 kDa Spin column (BioVision Cat# 1997).
What are the difference between the K606, K686 and K688 assay kit?
There are many differences between these 3 kits although they all measure the sample glucose concentration. A few of these differences which might make your decision of making a choice easier are:K606-100:Applicable for both colorimetric and fluorimetric detection.Requires filters for 570 nm and Ex/Em: 535/587 nm.Detetion limit of 1-10000 uM glucoseUtilizes the oxired probeK686-100Applicable only for colorimetric detection.Requires filters for 450 nm.Detetion limit of 20 uM to 10 mM glucoseDoes not utilize Oxired or Picoprobe (TM)Ideal for samples having reducing substancesK688-100Applicable only for fluorimetric detection.Requires filters for Ex/Em: 535/587 nm.Detetion limit of < 0.5 uM glucoseUtilizes the Picoprobe (TM)
Can this kit be used with samples like bacteria, plants, drosophila etc?
We have optimized the kit with mammalian samples. However, theoretically these kits should work with samples from multiple species/sources. Since the optimal conditions depend on the sample type, the protocol has to be be adapted to fit the samples for efficient results. Please refer to this kit's citations to see what kind of samples have been used with this kit other than mammalian samples.
Can we use frozen samples with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Can we use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Why are my standard curve values lower than those shown on the datasheet?
There are multiple factors which influence the signals like the incubation times, room temperature, handling etc. In general, to increase the value of the standards, you can increase the incubation time. As long as the standard curve is linear, it should be fine to use, since all of your samples will also be measured under the same conditions on this curve.
How do I normalize my samples against protein concentration
You can use a protein quantitation assay on the supernatants you get from cell/tissue lysates or with any other liquid sample in the assay buffer.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Should I make a standard curve for every expt I do, or is one curve/kit enough?
Yes, I would strongly recommend you to do the standards every time you do the expt. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.
Could you please provide a sample preparation protocol for cells and tissues?
This kit is ideal for serum and urine samples. For tissues and cells, homogenize 1 x 106 cell or 10 mg of tissue in 100 μl of assay buffer provided with the kit. You can either use a dounce homogenizer or sonicate on ice to homogenize and lyse the cells. Centrifuge the lysate at 10,000 g for 10 min and take the supernatant for the assay. Supernatant is then filtered through a 10kDa MW spin filter (BV Cat.# 1997-25) to remove all enzymes and proteins. Note: if you want to use more cells/tissues, increase the amount of assay buffer proportionally to make sure that the lysis is complete.
Mariana Guerra-Maupome, Aerosol vaccination with Bacille Calmette-Guerin induces a trained innate immune phenotype in calves. PLoS One, Feb 2019; 30794653.
Ruifang Gao, CD44ICD promotes breast cancer stemness via PFKFB4-mediated glucose metabolism. Theranostics, Nov 2018;  30613295.
Ling, Z. et al., (2017) miR-361-5p modulates metabolism and autophagy via the Sp1-mediated regulation of PKM2 in prostate cancer, Oncology Reports, Jul.2017, 38(3), 1621-1628
Li et al., Crucial role of TRPC6 in maintaining the stability of HIF-1α in glioma cells under hypoxia. J. Cell Sci., Sep 2015; 128: 3317 - 3329.
Shi et al., A Novel KLF4/LDHA Signaling Pathway Regulates Aerobic Glycolysis in and Progression of Pancreatic Cancer. Clin. Cancer Res., Aug 2014; 20: 4370 - 4380.
For more citations of this product click here