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Glucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay Kit

based on 4 citations in multiple journalsGlucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay Kit44.1 4
Catalog #: K757
$395.00

Product Details

Cat # +Size K757-100
Size 100 assays
Detection Method Absorbance (450 nm)
Applications Measurement of G6PDH activity in various tissues and cells
Evaluation of pentose phosphate pathway
Features & Benefits • Simple procedure; takes ~ 30-60 minutes
• Fast and convenient
Kit Components • G6PDH Assay Buffer
• G6PDH Substrate
• G6PDH Developer
• G6PDH Positive Control
• NADH Standard (0.5 µmol)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. BioVision's glucose-6-phosphate dehydrogenase Assay Kit is a simple, sensitive and rapid assay detects the activity of G6PDH in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product which is utilized to convert a nearly colorless probe to an intensely colored product with an absorbance at 450nm. The G6PDH Assay Kit can detect as low as 0.04mU G6PDH per well.


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What is the source of the positive control?
The positive control is a Leuconostoc mesenteroides enzyme purified from E.Coli.
Why is NADH used in this assay instead of NADPH? Can NADPH interfere with the assay?
The first step of the reaction where G6PDH converts G6P to the gluconolactone is a very specific reaction. The NADH formed in this reaction reacts with the probe to generate a colored product which is measured at 450nm. Since this step is very specific NADPH should not interfere with the assay.
Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Hung et al., A long noncoding RNA connects c-Myc to tumor metabolism. PNAS, Dec 2014; 111: 18697 - 18702.
Smith et al., Renal cortical hexokinase and pentose phosphate pathway activation through the EGFR/Akt signaling pathway in endotoxin-induced acute kidney injury. Am J Physiol Renal Physiol, Aug 2014; 307: F435 - F444.
I-Tung Chen, et. al.  White Spot Syndrome Virus Induces Metabolic Changes Resembling the Warburg Effect in Shrimp Hemocytes in the Early Stage of Infection. J. Virol., Dec 2011; 85: 12919 - 12928.
Vázquez-Medina, J. et. al. Prolonged fasting increases glutathione biosynthesis in postweaned northern elephant seals. J. Exp. Biol., Apr 2011; 214: 1294 - 1299.
For more citations of this product click here