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Glucose-6-Phosphate Colorimetric Assay Kit

based on 1 citations in multiple journalsGlucose-6-Phosphate Colorimetric Assay Kit14.1 4
Catalog #: K657

In stock

$395.00

Product Details

Cat # +Size K657-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian
Applications The Glucose-6-phosphate Assay Kit can detect G6P in the range of 1 to 30 nmoles with detection sensitivity ~10 µM of G6P.
Features & Benefits • Simple procedure; takes ~ 40 minutes
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Glucose-6-Phosphate (G6P)
Kit Components • G6P Assay Buffer
• G6P Enzyme Mix
• G6P Substrate Mix
• G6PStandard (10 µmol)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glucose-6-phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. BioVision's glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product which is utilized to convert a nearly colorless probe to an intensely colored product with an absorbance at 450 nm. The Glucose-6-phosphate Assay Kit can detect G6P in the range of 1 to 30 nmoles with detection sensitivity ~10 µM of G6P.


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Does this kit also detect G-1-P?
No, this kit is specific for G-6-P since the enzyme at the first step of the detection reaction only recognizes G6P as the substrate.
What is the range/sensitivity of this kit?
This kit can detect G6P in the range of 1 to 30 nmoles with detection sensitivity ~10 µM of G6P.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples since this is an enzyme activity assay. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Is it essential to make a standard curve for every expt, or is one curve/kit enough?
Yes, it is strongly recommended to do the standards every time you do the expt. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature (-20C) and in appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Shearer, J. et. al. Exercise training does not correct abnormal cardiac glycogen accumulation in the db/db mouse model of type 2 diabetes. Am J Physiol Endocrinol Metab, Jul 2011; 301: E31 - E39.
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