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Product Details
| Cat # +Size | K697-100 |
|---|---|
| Size | 100 assays |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Mammalian |
| Applications | The kit can detect glucose-1-phosphate concentrations in the range of 1 µM – 10 mM. |
| Features & Benefits | • Simple procedure; takes ~ less than 40 minutes • Fast and convenient |
| Kit Components | • G1P Assay Buffer• G1P Enzyme Mix (Lyophilized)• G1P Developer (Lyophilized)• G1P Substrate Mix (Lyophilized)• G1P Standard (Lyophilized) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose metabolism and storage. In response to hormonal or neural signals, glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen through the actions of Phosphoglucose isomerase (G6P G1P), UDPG-pyrophosphorylase (G1P UDP-glucose) and glycogen synthase (UDP-glucose + glycogen[n] UDP + glycogen[n+1]). Glucose-1-phosphate is found in virtually all organisms from bacteria to higher plants and animals. Measurement of intracellular G1P levels is crucial for analyzing the carbohydrate metabolic pathways and their kinetic properties. In Glucose-1-phosphate assay, G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a colorless probe to a colored product with strong absorbance at 450 nm. BioVision’s Glucose-1-phosphate assay kit is rapid, sensitive & easy to use & can detect 1µM to 10 mM G1P. This G1P assay kit can be used for a variety of sample types.
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Our test sample is yeast cells.Can this kit test yeast cells with the current protocol or not?Shoud we add a step to lyse the yeast cell use another cell lysis buffer before the testing?
Our kits are developed using mammalian cells which is why the assay buffer is not designed to lyse the yeast cell wall. We recommend using this for cell lysis: http://www.biovision.com/ezlys-yeast-protein-extraction-reagent-6799.html Glucose-1-P is not species specific and can be estimated in a variety of samples species using our kit.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Is it possible to use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. It is recommended to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Why are the standard curve values lower than those shown on the datasheet?
There are multiple factors which influence the signals like the incubation times, room temperature, handling etc. In general, to increase the value of the standards, the incubation time can be increased. As long as the standard curve is linear, it should be fine to use, since all the samples will also be measured under the same conditions on this curve.
How are samples normalized against protein concentration?
A protein quantitation assay can be used with the supernatants from cell/tissue lysates or with any other liquid sample in the assay buffer.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Guang-Zhi Jin, et al., ( 2018) Phosphoglucomutase 1 inhibits hepatocellular carcinoma progression by regulating glucose trafficking, PLoS Biology, Oct. 2018, 30335765
For more citations of this product click here
