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Glucokinase (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of glucokinase in human serum, plasma, culture supernatants and other biological fluids.
Catalog #: E4597

In stock

$695.00

Product Details

Cat # +Size E4597-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Human
• Application- quantitative measurement of glucokinase in human serum, plasma, tissue homogenates and other biological fluids.
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This ELISA kit is used for quantitative measurement of glucokinase in human serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits • Easy, convenient and time-saving method to measure the level of glucokinase in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• Detection Range: 0.781 - 50 ng/ml
• Sensitivity: < 0.469 ng/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10% No significant cross-reactivity or interference was observed.
Kit Components • Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glucokinase (GCK) is expressed in specific types of tissues: liver, pancreas, small intestine and brain. Glucokinase functions as a glucose sensor, triggering shifts in carbohydrate metabolism or cell function in response to the levels of glucose in blood, such as nutritional and hormonal molecular pathways. Unlike other Hexokinases, Glucokinase has a relatively low affinity for glucose and it is not inhibited by physiological concentrations of glucose 6-phosphate. Mutations in the gene encoding GCK can cause both hyperglycemia and hypoglycemia. Due to the major role of Glucokinase in controlling blood glucose homeostasis, Glucokinase is currently considered as a strong candidate target for the treatment of Hyperglycemia, a condition encountered in Type 2 Diabetic patients. BioVision’s Glucokinase ELISA kit is a sandwich ELISA assay for the quantitative measurement of Glucokinase in Human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Glucokinase captured from the samples.


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