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GFP Quantitation Kit

based on 2 citations in multiple journalsGFP Quantitation Kit24.1 4
Catalog #: K815
$340.00

Product Details

Cat # +Size K815-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 488/507 nm)
Species Reactivity Mammalian
Applications It detects a wide range of GFP concentration (0.01-10 ug/ml)
Features & Benefits • Simple procedure; takes ~ 20 minutes
• Fast and convenient
Kit Components • GFP Assay Buffer
• GFP Standard (1 µg/µl)
• GFP Quench Solution
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Green Fluorescent Protein (GFP) is a spontaneously fluorescent protein originally isolated from the jellyfish Aequorea Victoria (Chalfie et al, 1994). GFP has also been recombinant modified and enhanced (called EGFP). GFP and EGFP have been widely used as a valuable reporter molecule for in vivo visualization of gene expression events in a variety of cell types and organisms. Since GFP requires no additional substrates or cofactors, GFP fluorescence can be easily detected under fluorescence microscope. However, most imaging studies of GFP are only qualitative. Since quantitative analysis of GFP expression level in cells or tissues are more informative and have wide applications, BioVision has developed the GFP Quantification Kit which quantifies GFP in 96 micro-plate format. Cells or tissues can be homogenized directly in the GFP Assay Buffer. The quantity of GFP is determined by comparing its fluorescence with that of GFP standard. The kit can detect a wide range of GFP concentration (0.01-10 µg/ml). A GFP quench solution is also provided for determining auto-fluorescence of cell or tissue extracts. Each kit provides sufficient reagents to perform up to 100 assays, Ex/Em = 488/507 nm.


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Baets et al., Defects of mutant DNMT1 are linked to a spectrum of neurological disorders. Brain, Apr 2015; 138: 845 - 861.
Chenguang Fan. et.al Short N-terminal sequences package proteins into bacterial microcompartments PNAS, Apr 2010; 107: 7509 - 7514.
For more citations of this product click here