Gentamicin (serum/urine) ELISA Kit

A competitive ELISA kit for the quantitative measurement of Gentamicin in Serum, urine, milk and tissues (i.e. pork, liver, chicken, fish and shrimp)

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: K4315 | abID: ab285241

Product Details

abID ab285241
Cat # +Size K4315-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of Gentamicin in serum, urine, milk and tissues.
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications This ELISA kit is used for quantitative measurement of Gentamicin in serum, urine, milk and tissues.
Features & Benefits • Easy, convenient and time-saving method to measure the level of Gentamicin in tissues, milk and liquid samples.
• Detection Range: 0.3 - 24.3 ppb (ng/ml)
• Sensitivity: 0.3 ppb
• Detection limitation: 3 ppb for tissues, milk and liquid samples
• Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.
Kit Components • Micro ELISA Plate
• Gentamicin Standards
• HRP-conjugate
• Antibody
• TMB substrate
• Stop Solution
• Sample Diluent
• Wash Buffer (10X)
• Extraction Solution
• Conjugate Buffer
• Plate sealers
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Gentamicin, an aminoglycosidic antibiotic, has a broad spectrum towards most bacteria, particularly gram-negative species. It is widely used by veterinaries, has industrials applications as an additive and can be found on foodstuff consumed by animals. Its popularity is due to its broad antibacterial spectrum, efficacy and low cost. It is also known that unregulated and excessive use of this antibiotic causes negative effects in humans (i.e. neurological, hearing and renal damage). Due to its dangerous side effects, the FDA has established strict parameters that are considered safe for human consumption. Consequently, monitoring Gentamicin concentration amounts in animal-derived food is critical. Standard techniques/instruments (HPLC or GC-MS) are utilized to detect Gentamicin. However, these techniques are complex, expensive, laborious, and time-consuming. Immunoassay techniques, such as ELISAs are commonly preferred as a simple, reliable and rapid method for the quantification of Gentamicin in various samples. BioVision’s Gentamicin ELISA Kit is a competitive-based ELISA that can be used for the determination of this antibiotic in dairy products, mammalian tissues and human biofluids. This Gentamicin detection kit is fast, can be completed within 90 min, offers ready-to-use reagents and can detect Gentamicin within a range between 0.3 to 24.3 ppb (ng/ml).

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