Gentamicin ELISA Kit (ab285241)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.3 ng/ml - 24.3 ng/ml
- Sample type: Milk, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
Gentamicin ELISA Kit
See all Gentamicin kits -
Detection method
Colorimetric -
Sample type
Milk, Urine, Serum, Tissue -
Assay type
Competitive -
Sensitivity
0.3 ng/ml -
Range
0.3 ng/ml - 24.3 ng/ml -
Assay duration
Multiple steps standard assay -
Product overview
Gentamicin, an aminoglycosidic antibiotic, has a broad spectrum towards most bacteria, particularly gram-negative species. It is widelyused by veterinaries, has industrials applications as an additive and can be found on foodstuff consumed by animals. Its popularity is due to its broad antibacterial spectrum, efficacy and low cost. It is also known that unregulated and excessive use of this antibiotic causes negative effects in humans (i.e. neurological, hearing and renal damage). Due to its dangerous side effects, the FDA has established strict parameters that are considered safe for human consumption. Consequently, monitoring Gentamicin concentration amounts in animalderived food is critical. Standard techniques/instruments (HPLC or GC-MS) are utilized to detect Gentamicin. However, these techniques are complex, expensive, laborious, and time-consuming. Immunoassay techniques, such as ELISAs are commonly preferred as a simple, reliable and rapid method for the quantification of Gentamicin in various samples.
Gentamicin ELISA Kit (ab285241, K4315) is a competitive-based ELISA that can be used for the determination of this antibiotic in dairy products, mammalian tissues and human biofluids. This Gentamicin detection kit is fast, can be completed within 90 min, offers ready-to-use reagents and can detect Gentamicin within a range between 0.3 to 24.3 ppb (ng/ml).
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 96 tests 10X Wash Buffer II 1 x 50ml Antibody I 1 x 7ml Conjugate Buffer I 1 x 7.5ml Extraction Buffer IV 1 x 2ml Gentamicin Coated Plate 1 unit Gentamicin Standards (S0 - S5) 6 x 1ml Goat Anti-Mouse IgG HRP Conjugate I 1 x 10µl Microplate Sealing Film 4 units Sample Diluent II 1 x 20ml Stop Solution VIII 1 x 10ml TMB Substrate I 1 x 12ml -
Research areas
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Relevance
Gentamicin is an aminoglycoside antibiotic used to treat infections caused by many different types of bacteria. Gentamicin is usually administered by intravenous infusion or intramuscular injection. -
Alternative names
- Gentamicin
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab285241 has not yet been referenced specifically in any publications.