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Gamma Glutamyl Transferase (GGT) Activity Fluorometric Assay Kit

Catalog #: K785
$385.00

Product Details

Cat # +Size K785-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 365/460 nm)
Species Reactivity Mammalian
Applications This assay detects GGT activity as low as 0.02 mIU in sample.
Features & Benefits • The assay is sensitive, stable and high-throughput adaptable.
Kit Components • GGT Assay Buffer
• GGT Substrate
• GGT Positive Control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

The Gamma-GlutamylTransferase (GGT; EC 2.3.2.2) is an enzyme that transfers gamma-glutamyl functional groups. It is found in many tissues, the most notable one being the liver, and has significance in medicine as a diagnostic marker. BioVision’s Gamma-GlutamylTransferase Assay Kit provides a convenient tool for sensitive detection of the GGT in a variety of samples. The GGT in sample will recognize L-γ-Glutamyl-AMC as a specific substrate leading to proportional fluorescence development. The activity of GGT can be easily quantified fluorometrically (Ex/Em = 365/460 nm). This assay detects GGT activity as low as 0.02 mIU in sample.


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Please provide recommended protocols for plasma sample preparation. Will EDTA interfere with the assay performance?
Here’s the recommended protocol for plasma sample preparation: Collect blood using EDTA as an anticoagulant. (EDTA is fine, don’t use heparin or citrate). Centrifuge the blood at 700-1000xg for 10’ at room temperature. Pipette off the top yellow plasma layers without disturbing the white buffy layer. You might need to dilute the plasma sample 1:2 with assay buffer if required. Add the plasma (diluted or undiluted) directly into each well. Adjust volume to 50ul with GGT assay buffer.