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Gamma Glutamyl Transferase (GGT) Activity Colorimetric Assay Kit

based on 1 citations in multiple journalsGamma Glutamyl Transferase (GGT) Activity Colorimetric Assay Kit14.1 4
Catalog #: K784
$445.00

Product Details

Cat # +Size K784-100
Size 100 assays
Detection Method Absorbance (418 nm)
Species Reactivity Mammalian
Applications This assay detects GGT activity as low as 0.5 mIU.
Features & Benefits • The assay is sensitive, stable and high-throughput adaptable.
Kit Components • GGT Assay Buffer
• GGT Substrate
• GGT Positive Control
• pNA Standard (2mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

The Gamma-GlutamylTransferase (GGT; EC 2.3.2.2) is an enzyme that transfers gamma-glutamyl functional groups. It is found in many tissues, the most notable one being the liver, and has significance in medicine as a diagnostic marker. BioVision’s Gamma-GlutamylTransferase Assay Kit provides a convenient tool for sensitive detection of the GGT in a variety of samples. The GGT in sample will recognize L-γ-Glutamyl-pNA as a specific substrate leading to proportional color development. The activity of GGT can be easily quantified colorimetrically (λ = 418 nm). This assay detects GGT activity as low as 0.5 mIU.


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Can it be used with plasma samples?
The kit can work with Plasma samples too. Here’s a protocol to process the samples: Collect blood sample in an EDTA containing tube. Centrifuge for 10 minutes at 1000 x g within 30 minutes of collection. Pipette off the top yellow plasma layer without disturbing the white buffy layer. Assay immediately or store plasma samples at < -20° C. Avoid repeated freeze/thaw cycles.
Andre Apostolatos, Preclinical testing of 5-amino-1-((1R,2S,3S,4R)-2,3-dihydroxy-4-methylcyclopentyl)-1H-imidazole-4-carboxamide: a potent protein kinase C-ι inhibitor as a potential prostate carcinoma therapeutic. Anticancer drugs, Jan 2019; 30204596.
Adler et al., A Quantitative Approach to Screen for Nephrotoxic Compounds In Vitro. J. Am. Soc. Nephrol., Aug 2015; 10.1681/ASN.2015010060.
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