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Galectin-3 (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of Galectin-3 in human serum, plasma, tissue lysates, and other biological fluids
Catalog #: E4883
$725.00

Product Details

Cat # +Size E4883-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications This Sandwich ELISA kit is designed to quantitatively measure amount of Galectin-3 in human serum, plasma, tissue lysates and other biological fluids
Features & Benefits • Detection range: 0.156 - 10 ng/ml
• This Sandwich ELISA is highly sensitive and highly specific for the detection of Galectin-3 in human samples. There is no significant cross-reactivity or interference between Galectin-3 and analogues
• Recovery range: between 85 - 105% for normal human serum and plasma samples
• Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
• Sensitivity: 0.094 ng/ml
Kit Components • ELISA Microplate
• Standard (Lyophilized) (10 ng)
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate Solution
• Stop Solution
• Wash Buffer (25X)
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Galectin-3 belongs to the family of β-galactoside-binding lectins and is ubiquitously expressed in human tissues such as kidneys, blood vessels, heart, and a variety of cell types. The protein is predominantly present in the cytoplasm, but it shuttles to the nucleus. Additionally, it is present on cell surfaces and secreted into circulation. Due to the different locations of the protein, it performs a variety of functions. In the nucleus, Galectin-3 is involved in RNA splicing and transcription of genes, while in the cytoplasm, it functions in cell survival. Galectin-3 also plays a significant role in biological activities such as inflammation, angiogenesis, host defense, and apoptosis. BioVision’s Galectin-3 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of Galectin-3 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed away with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Galectin-3 in the sample or standard.


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