Galactose Colorimetric/Fluorometric Assay Kit

Sensitive Assay, HTS
Catalog #: K621 | abID: ab83382

Product Details

abID ab83382
Cat # +Size K621-100
Size 100 assays
Detection Method Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications N/A
Features & Benefits • Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Galactose
Kit Components • Galactose Assay Buffer
• Galactose Probe (DMSO solution)
• Galactose Enzyme Mix (Lyophilized)
• HRP (Lyophilized)
• Galactose Standard (100 nmol/µl)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Galactose (C₆H₁₂O₆ FW: 180.16) is one the major naturally occurring sugars, involved in many biological processes. BioVision’s Galactose Assay Kit provides a simple, convenient means for direct measurement of galactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). In the assay kit, Galactose is specifically oxidized generating a product that produces color (O.D 570 nm) and fluorescence (Ex/Em 535/587 nm). Liquid samples can be tested directly without purification. The assay is fast, convenient and sensitive. It can also be used as a high throughput assay.

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Can this kit can distinguish between lactose and galactose and can it distinguish between galactose and its derivatives (gal-1 phosphate, UDP-galactose, etc)?
The enzyme used in the assay is specific for the oxidation of galactose and it will not react with lactose or derivatives of galactose.
Is this kit only for the detection of galactose monomer and it will not detect galactose in disaccharides for example?
This kit detects Galactose as a monosaccharide. Galactan, the polymer of galactose found in hemicellulose can be converted to galactose by hydrolysis and then used with this kit. We have not tested Galactan or galactose polymers with this kit.
Is only free galactose measurable or protein-bound galactose is also measured?
The galactose has to be free and oxidizable to be measured by this kit.
Can galactosidase activity be measured by this kit?
No, this kit measures Galactose and not enzyme activity of Galactosidase.
Can 100 test samples be used with this kit?
A total of 100 reactions can be run including samples, standards and controls using the reagents provided in this kit.
Can protein content be used as an internal control for this assay?
Yes, a detergent-compatible BCA assay can be used for protein quantitation to normalize sample amount.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted."
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Is it essential to make a standard curve for every expt, or is one curve/kit enough?
Yes, it is essential to do the standards every time you do the expt to ensure there is no absorption of ethanol vapors into the reagents. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.
Young, Bridget E. et al. (2017) Markers of Oxidative Stress in Human Milk do not Differ by Maternal BMI But are Related to Infant Growth Trajectories, Matern Child Health J. 2017 Jun;21(6):1367-1376.
David A. Sela et al.,  Bifidobacterium longum subsp. infantis ATCC 15697 α-Fucosidases Are Active on Fucosylated Human Milk Oligosaccharides, Appl. Envir. Microbiol., Feb 2012; 78: 795 - 803.
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