Furin Activity Assay Kit (Fluorometric) (ab289844)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Plasma, Serum
Overview
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Product name
Furin Activity Assay Kit (Fluorometric)
See all Furin kits -
Detection method
Fluorescent -
Sample type
Serum, Plasma -
Assay type
Enzyme activity -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mammals -
Product overview
This Furin Activity Assay Kit (ab289844, K2076) provides a quick, easy, and sensitive method to determine Furin activity in various samples. In this assay, Furin activity is measured in the presence or absence of Furin Inhibitors. The difference between the fluorescence obtained in the presence or absence of Furin Inhibitors represents the actual Furin activity. A potent, specific Furin inhibitor is also included in the kit.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests AMC Standard 1 x 100µl Deactivator (50 mM) 1 x 200µl Furin Assay Buffer 1 x 25ml Furin Inhibitor (1 mM) 1 x 25µl Furin Positive Control 1 x 10µl Furin Substrate 1 x 25µl -
Research areas
Images
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Example data of an AMC Standard Curve. A new standard curve should be generated alongside each assay.
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Kinetic activity of Furin Activity in human serum samples including one Covid-19 negative donor serum and two Covid-19 positive donor serums respectively. The results are presented as ΔRFU, fluorescence detected in the presence of the inhibitor is subtracted from the fluorescence detected in the absence of the inhibitor.
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Furin Activity in human serum samples spiked with different amount of Furin Positive Control. The average spike recoveries were 90.94%, 92.05% and 114.78%, respectively. Assay was performed following the kit protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab289844 has not yet been referenced specifically in any publications.