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Fumonisin B1 ELISA Kit

Competitive ELISA kit for the quantitative measurement of Fumonisin B1 in grain, peanut and feed samples.
Catalog #: E4749
$525.00

Product Details

Cat # +Size E4749-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications • Detection limit: Corn, feed - 50 ppb, Edible oil - 10 ppb
• Sample recovery rate: Feed - 95%±15%, Corn - 100%±15%, Edible oil - 85%±15%
• Cross-reactivity: Fumonisin B1 (FB1) -100%
• Quantitative measurement of Fumonisin B1 in grain, peanut and feed samples.
• Sensitivity: 0.5 ppb (ng/mL)
Features & Benefits • Sensitivity: 0.5 ppb (ng/mL)
• Cross-reactivity: Fumonisin B1 (FB1) -100%
• Quantitative measurement of Fumonisin B1 in grain, peanut and feed samples.
• Sample recovery rate: Feed - 95%±15%, Corn - 100%±15%, Edible oil - 85%±15%
• Detection limit: Corn, feed - 50 ppb, Edible oil - 10 ppb
Kit Components Substrate Reagent A
Standard
Plate Sealer
Reconstitution Buffer (10x)
ELISA Microplate
Wash Buffer (20X)
Antibody Working Solution
Stop Solution
Substrate Reagent B
HRP Conjugate
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Fumonisin B1 is the most prevalent member of a family of toxins, known as fumonisins, produced by several species of Fusarium molds, such as Fusarium verticillioides, which occur mainly in maize (corn), wheat and other cereals. Fumonisin B1 is hepatotoxic and nephrotoxic in all animal species tested. The earliest histological change to appear in either the liver or kidney of fumonisin-treated animals is increased apoptosis followed by regenerative cell proliferation. BioVision’s fumonisin B1 ELISA Kit is based on Indirect-Competitive-ELISA method. It can detect fumonisin B1 (FB1) in samples, such as grain, peanut, feed, etc. The Microtiter plate provided in this kit has been pre-coated with FB1. During the reaction, FB1 in the samples or standard competes with FB1on the solid phase supporter for sites of FB1 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microplate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of FB1. The concentration of FB1 in the samples can be calculated by comparing the OD of the samples to the standard curve.


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