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Fructose Colorimetric/Fluorometric Assay Kit

based on 2 citations in multiple journalsFructose Colorimetric/Fluorometric Assay Kit24.1 4
Sensitive Assay, HTS
Catalog #: K619

Product Details

Cat # +Size K619-100
Size 100 assays
Detection Method Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications N/A
Features & Benefits • Simple procedure; takes ~ 1 hour
• Fast and convenient
• The sensitive kit contains all necessary reagents for accurate measurement of Fructose
Kit Components • Fructose Assay Buffer
• OxiRed Probe (in DMSO)
• Enzyme Mix
• Fructose Converting Enzyme
• Fructose Standard (100mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose. In BioVision’s Fructose Assay Kit, free fructose is enzymatically converted to β-glucose, which is then specifically converted to a product that reacts with OxiRed Probe to generate color (λ=570nm) and fluorescence (Ex/Em=535/587nm). The kit provides a rapid, simple, sensitive, and reliable method suitable for high throughput assayof D-fructose.

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Since the converting enzyme is unstable, will storing it at -20C after use increase its shelf-life?
The converting enzyme is unstable when not in ammonium sulphate solution. It is recommended to store it at 4C after initial thaw and use it up within 2 months. Freezing and thawing even when stored in aliquots might decrease its activity dramatically. Hence storing it at -20C after first thaw is not recommended.
The glucose background in the samples is very high and the RFU measured is in 100,000 range. What is the best way to deal with this issue?
When doing the fluorometric assay it is important to use 0.4ul probe to reduce background. Also, the fluorometer sensitivity setting should be at "medium" or "low" so that noise does not get amplified.
Can this kit be used with samples from fruits?
We have optimized the kit with mammalian samples. However, theoretically these kits should work with samples from multiple species/sources including fruits. Since the optimal conditions depend on the sample type, the protocol has to be be adapted to fit the samples for efficient results. Please refer to this kit's citations to see what kind of samples have been used with this kit other than mammalian samples.
Will alpha glucose interfere in this assay?
Alpha glucose typically makes up polymers like starch and should not interfere in this assay.
Can we use frozen samples with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Jianghai Liu et al., Upregulation of aldolase B and overproduction of methylglyoxal in vascular tissues from rats with metabolic syndrome, Cardiovasc Res, Dec 2011; 92: 494 - 503.
Liu, J. et. al. Upregulation of aldolase B and overproduction of methylglyoxal in vascular tissues from rats with metabolic syndrome. Cardiovasc Res, Sep 2011; 10.1093/cvr/cvr239.
For more citations of this product click here