Product Details
abID | ab65341 |
---|---|
Cat # +Size | K612-100 |
Size | 100 assays |
Detection Method | Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) |
Species Reactivity | Mammalian |
Applications | The kit has a detection limit 2 µM free fatty acid in variety samples. |
Features & Benefits | • Simple procedure; takes ~40 minutes • Fast and convenient • Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples |
Kit Components | • Fatty Acid Assay Buffer • Fatty Acid Probe (in DMSO) • ACS Reagent • Enzyme Mix • Enhancer • Palmitic Acid Standard (1nmol/µl) |
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |
Details
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a. The lower limit of detection is calculated by taking 2 nmol (lowest conc of Standard) divided by 50 µl (max sample volume)=2 nmol/50µl= 0.0.04 nmol/µl
b. The highest limit of detection is calculated by taking 10 nmol (highest conc of Standard) divided by 1 µl (min sample volume)=10 nmol/1 µl= 10 nmol/µl